tailieunhanh - Báo cáo y học: " Mucin granule-associated proteins in human bronchial epithelial cells: the airway goblet cell “granulome”

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Mucin granule-associated proteins in human bronchial epithelial cells: the airway goblet cell “granulome”. | Raiford et al. Respiratory Research 2011 12 118 http content 12 1 118 RESPIRATORY RESEARCH RESEARCH Open Access Mucin granule-associated proteins in human bronchial epithelial cells the airway goblet cell granulome 2 13 1 1 1 Kimberly L Raiford Joungjoa Park Ko-Wei Lin Shijing Fang Anne L Crews and Kenneth B Adler Abstract Background Excess mucus in the airways leads to obstruction in diseases such as chronic bronchitis asthma and cystic fibrosis. Mucins the highly glycosolated protein components of mucus are stored in membrane-bound granules housed in the cytoplasm of airway epithelial goblet cells until they are secreted into the airway lumen via an exocytotic process. Precise mechanism s of mucin secretion including the specific proteins involved in the process have yet to be elucidated. Previously we have shown that the Myristoylated Alanine-Rich C Kinase Substrate MARCKS protein regulates mucin secretion by orchestrating translocation of mucin granules from the cytosol to the plasma membrane where the granules dock fuse and release their contents into the airway lumen. Associated with MARCKS in this process are chaperone Heat Shock Protein 70 HSP70 Cysteine string protein CSP and cytoskeletal actin myosin proteins. However additional granule-associated proteins that may be involved in secretion have not yet been elucidated. Methods Here we isolated mucin granules and granule membranes from primary cultures of well differentiated human bronchial epithelial cells utilizing a novel technique of immuno-isolation based on the presence of the calcium activated chloride channel hCLCA1 the human ortholog of murine Gob-5 on the granule membranes and verified via Western blotting and co-immunoprecipitation that MARCKS HSP70 CSP and hCLCA1 were present on the granule membranes and associated with each other. We then subjected the isolated granules membranes to liquid chromatography mass spectrometry LC-MS MS to identify other granule associated .