tailieunhanh - Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates . | Kim et al. Respiratory Research 2011 12 108 http content 12 1 108 RESPIRATORY RESEARCH RESEARCH Open Access Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates Escherichia coli-induced acute lung injury in mice 1 f 3 111 2 Eun Sun Kim Yun Sil Chang Soo Jin Choi Jin Kyu Kim Hey Soo Yoo So Yoon Ahn Dong Kyung Sung Soo Yoon Kim2 Ye Rim Park2 and Won Soon Park1 2 Abstract Background Human umbilical cord blood UCB -derived mesenchymal stem cells MSCs attenuate hyperoxic neonatal lung injury primarily through anti-inflammatory effects. We hypothesized that intratracheal transplantation of human UCB-derived MSCs could attenuate Escherichia coli E. coli -induced acute lung injury ALI in mice by suppressing the inflammatory response. Methods Eight-week-old male ICR mice were randomized to control or ALI groups. ALI was induced by intratracheal E. coli instillation. Three-hours after E. coli instillation MSCs fibroblasts or phosphate-buffered saline were intratracheally administered randomly and survival was analyzed for 7 days post-injury. Lung histology including injury scores myeloperoxidase MPO activity and protein levels of interleukin IL -1a IL-1P IL-6 tumor necrosis factor TNF -a and macrophage inflammatory protein MIP -2 as well as the wet-dry lung ratio and bacterial counts from blood and bronchoalveolar lavage BAL were evaluated at 1 3 and 7 days post-injury. Levels of inflammatory cytokines in the lung were also profiled using protein macroarrays at day 3 post-injury which showed peak inflammation. Results MSC transplantation increased survival and attenuated lung injuries in ALI mice as evidenced by decreased injury scores on day 3 post-injury and reduced lung inflammation including increased MPO activity and protein levels of IL-1a IL-1P IL-6 TNF-a and MIP-2 on day 3 and 7 post-injury. Inflammatory cytokine profiles in the lungs at day 3 post-injury were attenuated by MSC .