tailieunhanh - Báo cáo y học: "Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus. | Horvath et al. Respiratory Research 2011 12 102 http content 12 1 102 RESPIRATORY RESEARCH RESEARCH Open Access Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus I I z-x 1 1 I -X -X I I zx zl-x I r2 3 I I -X 11-xzx 7 I zx I 13 4 I I zx XX zx 4 zx 7 I -X X- 4 I zx z zx ỉ I I r -X I 2 3 -X XX z-l I I zx XX -X I -X r x-xzx 1 2 3 Katherine M Horvath Margaret Herbst Haibo Zhou Hongtao Zhang lerry L Noah and Ilona Jaspers Abstract Background Modified function of immune cells in nasal secretions may play a role in the enhanced susceptibility to respiratory viruses that is seen in smokers. Innate immune cells in nasal secretions have largely been characterized by cellular differentials using morphologic criteria alone which have successfully identified neutrophils as a significant cell population within nasal lavage fluid NLF cells. However flow cytometry may be a superior method to fully characterize NLF immune cells. We therefore characterized immune cells in NLF by flow cytometry determined the effects of live attenuated influenza virus LAIV on NLF and peripheral blood immune cells and compared responses in samples obtained from smokers and nonsmokers. Methods In a prospective observational study we characterized immune cells in NLF of nonsmokers at baseline using flow cytometry and immunohistochemistry. Nonsmokers and smokers were inoculated with LAIV on day 0 and serial nasal lavages were collected on days 1-4 and day 9 post-LAIV. LAIV-induced changes of NLF cells were characterized using flow cytometry. Cell-free NLF was analyzed for immune mediators by bioassay. Peripheral blood natural killer NK cells from nonsmokers and smokers at baseline were stimulated in vitro with LAIV followed by flow cytometric and mediator analyses. Results CD45 CD56 - CD16 neutrophils and CD45 CD56 NK cells comprised median range and respectively of non-squamous NLF cells in .