tailieunhanh - Báo cáo y học: " Angiogenesis gene expression in murine endothelial cells during post-pneumonectomy lung growth"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Angiogenesis gene expression in murine endothelial cells during post-pneumonectomy lung growth | Lin et al. Respiratory Research 2011 12 98 http content 12 1 98 RESPIRATORY RESEARCH RESEARCH Open Access Angiogenesis gene expression in murine endothelial cells during post-pneumonectomy lung growth 1111 12 Miao Lin Kenji Chamoto Barry C Gibney Grace S Lee Dinee Collings-Simpson Jan Houdek Moritz A Konerding2 Akira Tsuda3 and Steven J Mentzer1 Abstract Although blood vessel growth occurs readily in the systemic bronchial circulation angiogenesis in the pulmonary circulation is rare. Compensatory lung growth after pneumonectomy is an experimental model with presumed alveolar capillary angiogenesis. To investigate the genes participating in murine neoalveolarization we studied the expression of angiogenesis genes in lung endothelial cells. After left pneumonectomy the remaining right lung was examined on days 3 6 14 and 21days after surgery and compared to both no surgery and sham thoracotomy controls. The lungs were enzymatically digested and CD31 endothelial cells were isolated using flow cytometry cell sorting. The transcriptional profile of the CD31 endothelial cells was assessed using quantitative real-time polymerase chain reaction PCR arrays. Focusing on 84 angiogenesis-associated genes we identified 22 genes with greater than 4-fold regulation and significantly enhanced transcription p .05 within 21 days of pneumonectomy. Cluster analysis of the 22 genes indicated that changes in gene expression did not occur in a single phase but in at least four waves of gene expression a wave demonstrating decreased gene expression more than 3 days after pneumonectomy and 3 sequential waves of increased expression on days 6 14 and 21 after pneumonectomy. These findings indicate that a network of gene interactions contributes to angiogenesis during compensatory lung growth. Introduction In most circumstances angiogenesis does not occur in the adult pulmonary circulation 1 2 . Although structural adaptations are well-documented in the bronchial .

TÀI LIỆU LIÊN QUAN
TỪ KHÓA LIÊN QUAN
crossorigin="anonymous">
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.