tailieunhanh - Báo cáo y học: " TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by. | Respiratory Research BioMed Central Research TGF-p1 induced epithelial to mesenchymal transition EMT in human bronchial epithelial cells is enhanced by IL-1 p but not abrogated by corticosteroids Astrid M Doerner1 and Bruce L Zuraw 1 2 Open Access Address Veterans Medical Research Foundation La Jolla California USA and 2Department of Medicine University of California San Diego La Jolla California USA Email Astrid M Doerner-adoerner@ Bruce LZuraw - bzuraw@ Corresponding author Published 27 October 2009 Received 22 November 2008 Respiratory Research 2009 10 100 doi 1465-9921-10-100 Accepted 27 October 2009 This article is available from http content 10 1 100 2009 Doerner and Zuraw licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Chronic persistent asthma is characterized by ongoing airway inflammation and airway remodeling. The processes leading to airway remodeling are poorly understood and there is increasing evidence that even aggressive anti-inflammatory therapy does not completely prevent this process. We sought to investigate whether TGFP1 stimulates bronchial epithelial cells to undergo transition to a mesenchymal phenotype and whether this transition can be abrogated by corticosteroid treatment or enhanced by the pro-inflammatory cytokine IL-1 p. Methods BEAS-2B and primary normal human bronchial epithelial cells were stimulated with TGFP1 and expression of epithelial and mesenchymal markers assessed by quantitative real-time PCR immunoblotting immunofluorescence microscopy and zymography. In some cases the epithelial cells were also incubated with corticosteroids or IL-1p. Results were analyzed using nonparametric statistical tests. Results .

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