tailieunhanh - Báo cáo y học: " Micro-computed tomography of pulmonary fibrosis in mice induced by adenoviral gene transfer of biologically active transforming growth factor-b1"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Micro-computed tomography of pulmonary fibrosis in mice induced by adenoviral gene transfer of biologically active transforming growth factor-b1. | Rodt et al. Respiratory Research 2010 11 181 http content 11 1 181 RESPIRATORY RESEARCH RESEARCH Open Access Micro-computed tomography of pulmonary fibrosis in mice induced by adenoviral gene transfer of biologically active transforming growth factor-p1 I I f r-rx -X f- D rx z-l 1 2r l r II r I -X rx X c -X I lz1 2 c -X tx I rx I Ì -rx r 1 D rx -X -x I_I -X I - -X r2 Dzx S I -x -X M X If3 1ZI zxC I I Ar I 4 A A X rx I rx I tx4 momas Rodt Christian von raiGK Sabine L ettmer Roman Halter Regina Maus Kjetn ask Martin ixoib Hr-lz AAi ildia4 rlcrne I li-rici 5 I I ir ir i I Izxw6 lA dltd7 t. licheo AAl ncH 1 I IIrlcR A AAni ic3t h lrrioe Rzxrdz23 Jack Gauidie rioiian Langei Ludwig Hoy Tobias vvene Micnaei GaiansKi omen A Maus Jdigen DOiiaK Abstract Background Micro-computed tomography micro-CT is a novei tooi for monitoring acute and chronic disease states in smaII Iaboratory animais. Its vaiue for assessing progressive Iung fibrosis in mice has not been reported so far. Here we examined the importance of in vivo micro-CT as non-invasive tooi to assess progression of puimonary fibrosis in mice over time. Methods Puimonary fibrosis was induced in mice by intratracheai deiivery of an adenovirai gene vector encoding bioiogicaiiy active TGF-R1 AdTGF-R1 . Respiratory gated and ungated micro-CT scans were performed at 1 2 3 and 4 weeks post puimonary adenovirai gene or controi vector deiivery and were then correiated with respective histopathoiogy-based Ashcroft scoring of puimonary fibrosis in mice. VisuaI assessment of image quaiity and consoiidation was performed by 3 observers and a semi-automated quantification aigorithm was appiied to quantify aerated puimonary voiume as an inverse surrogate marker for puimonary fibrosis. Results We found a significant correiation between ciassicai Ashcroft scoring and micro-CT assessment using both visuai assessment and the semi-automated quantification aigorithm. Puimonary fibrosis couid be cieariy .