tailieunhanh - Báo cáo y học: " Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease. | Hackett et al. Respiratory Research 2010 11 180 http content 11 1 180 RESPIRATORY RESEARCH RESEARCH Open Access Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease 3 2 2 T- T- 3 1 Tillie L Hackett 1 Marco Scarci Lu Zheng Wan Tan Tom Treasure Jane A Warner Abstract Background There is accumulating evidence that oxidative stress plays an important role in the pathophysiology of chronic obstructive pulmonary disease COPD . One current hypothesis is that the increased oxidant burden in these patients is not adequately counterbalanced by the lung antioxidant systems. Objective To determine the levels of oxidised human serum albumin HSA in COPD lung explants and the effect of oxidation on HSA degradation using an ex vivo lung explant model. Methods Parenchymal lung tissue was obtained from 38 patients 15F 23M undergoing lung resection and stratified by smoking history and disease using the GOLD guidelines and the lower limit of normal for FEV1 FVC ratio. Lung tissue was homogenised and analysed by ELISA for total levels of HSA and carbonylated HSA. To determine oxidised HSA degradation lung tissue explants were incubated with either 200 pg ml HSA or oxidised HSA and supernatants collected at 1 2 4 6 and 24 h and analysed for HSA using ELISA and immunoblot. Results When stratified by disease lung tissue from GOLD II median pg ml and GOLD I median pg ml patients had lower levels of HSA compared to patients with normal lung function median pg ml P . In addition the number of carbonyl residues which is a measure of oxidation was elevated in GOLD I and II tissue compared to individuals with normal lung function P . When analysing smoking status current smokers had lower levels of HSA median pg ml P compared to ex smokers median pg ml and non-smokers median pg ml and significantly greater number of carbonyl residues per HSA .