tailieunhanh - Báo cáo y học: " Estrogen aggravates inflammation in Pseudomonas aeruginosa pneumonia in cystic fibrosis mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Estrogen aggravates inflammation in Pseudomonas aeruginosa pneumonia in cystic fibrosis mice. | Wang et al. Respiratory Research 2010 11 166 http content 11 1 166 RESEARCH RESPIRATORY RESEARCH Open Access Estrogen aggravates inflammation in Pseudomonas aeruginosa pneumonia in cystic fibrosis mice Yufa Wang 1 Elvis Cela1 Stéphane Gagnon1 Neil B Sweezey1 2 Abstract Background Among patients with cystic fibrosis CF females have worse pulmonary function and survival than males primarily due to chronic lung inflammation and infection with Pseudomonas aeruginosa P. aeruginosa . A role for gender hormones in the causation of the CF gender gap has been proposed. The female gender hormone 17p-estradiol E2 plays a complex immunomodulatory role in humans and in animal models of disease suppressing inflammation in some situations while enhancing it in others. Helper T-cells were long thought to belong exclusively to either T helper type 1 Th1 or type 2 Th2 lineages. However a distinct lineage named Th17 is now recognized that is induced by interleukin IL -23 to produce IL-17 and other pro-inflammatory Th17 effector molecules. Recent evidence suggests a central role for the IL-23 IL-17 pathway in the pathogenesis of CF lung inflammation. We used a mouse model to test the hypothesis that E2 aggravates the CF lung inflammation that occurs in response to airway infection with P. aeruginosa by a Th17-mediated mechanism. Results Exogenous E2 caused adult male CF mice with pneumonia due to a mucoid CF clinical isolate the P. aeruginosa strain PA508 PA508 to develop more severe manifestations of inflammation in both lung tissue and in bronchial alveolar lavage BAL fluid with increased total white blood cell counts and differential and absolute cell counts of polymorphonuclear leukocytes neutrophils . Inflammatory infiltrates and mucin production were increased on histology. Increased lung tissue mRNA levels for IL-23 and IL-17 were accompanied by elevated protein levels of Th17-associated pro-inflammatory mediators in BAL fluid. The burden of PA508 .