tailieunhanh - Báo cáo y học: " Elemental analysis of lung tissue particles and intracellular iron content of alveolar macrophages in pulmonary alveolar proteinosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Elemental analysis of lung tissue particles and intracellular iron content of alveolar macrophages in pulmonary alveolar proteinosis. | Shimizu et al. Respiratory Research 2011 12 88 http content 12 1 88 RESPIRATORY RESEARCH RESEARCH Open Access Elemental analysis of lung tissue particles and intracellular iron content of alveolar macrophages in pulmonary alveolar proteinosis Yasuo Shimizu1 2 Shinichi Matsuzaki1 Kunio Dobashi3 Noriko Yanagitani1 Takahiro Satoh4 Masashi Koka4 Akihito Yokoyama4 Takeru Ohkubo4 Yasuyuki Ishii4 Tomihiro Kamiya4 and Masatomo Mori1 Abstract Background Pulmonary alveolar proteinosis PAP is a rare disease occurred by idiopathic autoimmune or secondary to particle inhalation. The in-air microparticle induced X-ray emission in-air micro-PIXE system performs elemental analysis of materials by irradiation with a proton microbeam and allows visualization of the spatial distribution and quantitation of various elements with very low background noise. The aim of this study was to assess the secondary PAP due to inhalation of harmful particles by employing in-air micro-PIXE analysis for particles and intracellular iron in parafin-embedded lung tissue specimens obtained from a PAP patient comparing with normal lung tissue from a non-PAP patient. The iron inside alveolar macrophages was stained with Berlin blue and its distribution was compared with that on micro-PIXE images. Results The elements composing particles and their locations in the PAP specimens could be identified by in-air micro-PIXE analysis with magnesium Mg aluminum Al silicon Si phosphorus P sulfur S scandium Sc potassium K calcium Ca titanium Ti chromium Cr copper Cu manganase Mn iron Fe and zinc Zn being detected. Si was the major component of the particles. Serial sections stained by Berlin blue revealed accumulation of sideromacrophages that had phagocytosed the particles. The intracellular iron content of alveolar macrophage from the surfactant-rich area in PAP was higher than normal lung tissue in control lung by both in-air micro-PIXE analysis and Berlin blue staining. Conclusion The .