tailieunhanh - Báo cáo y học: " Immunohistochemical detection and regulation of a5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Immunohistochemical detection and regulation of a5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis. | Porter et al. Respiratory Research 2011 12 82 http content 12 1 82 RESPIRATORY RESEARCH RESEARCH Open Access Immunohistochemical detection and regulation of a5 nicotinic acetylcholine receptor nAChR subunits by FoxA2 during mouse lung organogenesis Jason L Porter Benjamin R Bukey Alex J Geyer Charles P Willnauer and Paul R Reynolds Abstract Background a5 nicotinic acetylcholine receptor nAChR subunits structurally stabilize functional nAChRs in many non-neuronal tissue types. The expression of a5 nAChR subunits and cell-specific markers were assessed during lung morphogenesis by co-localizing immunohistochemistry from embryonic day E to post natal day PN 20. Transcriptional control of a5 nAChR expression by FoxA2 and GATA-6 was determined by reporter gene assays. Results Steady expression of a5 nAChR subunits was observed in distal lung epithelial cells during development while proximal lung expression significantly alternates between abundant prenatal expression absence at PN4 and PN10 and a return to intense expression at PN20. a5 expression was most abundant on luminal edges of alveolar type AT I and ATII cells non-ciliated Clara cells and ciliated cells in the proximal lung at various periods of lung formation. Expression of a5 nAChR subunits correlated with cell differentiation and reporter gene assays suggest expression of a5 is regulated in part by FoxA2 with possible cooperation by GATA-6. Conclusions Our data reveal a highly regulated temporal-spatial pattern of a5 nAChR subunit expression during important periods of lung morphogenesis. Due to specific regulation by FoxA2 and distinct identification of a5 in alveolar epithelium and Clara cells future studies may identify possible mechanisms of cell differentiation and lung homeostasis mediated at least in part by a5-containing nAChRs. Keywords alpha 5 development epithelium lung nAChR Background Pulmonary development adheres to orchestrated processes that require precisely .