tailieunhanh - Báo cáo y học: " A promoter SNP rs4073TA in the common allele of the interleukin 8 gene is associated with the development of idiopathic pulmonary fibrosis via the IL-8 protein enhancing mode"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " A promoter SNP rs4073TA in the common allele of the interleukin 8 gene is associated with the development of idiopathic pulmonary fibrosis via the IL-8 protein enhancing mode. | Ahn et al. Respiratory Research 2011 12 73 http content 12 1 73 RESPIRATORY RESEARCH RESEARCH Open Access A promoter SNP rs4073T A in the common allele of the interleukin 8 gene is associated with the development of idiopathic pulmonary fibrosis via the IL-8 protein enhancing mode 1 f 2f 1 1 111 Mi-Hyun Ahn Byung-Lae Park Shin-Hwa Lee Sung-Woo Park Jong-Sook Park Do-Jin Kim An-Soo Jang Jai-Soung Park3 Hwa-Kyun Shin4 Soo-Taek Uh5 Yang-Ki Kim5 Young Whan Kim6 Sung Koo Han6 Ki-Suck Jung7 8 9 9 10 10 11 Kye Young Lee Sung Hwan Jeong Jeong Woong Park Byoung Whui Choi In Won Park Man Pyo Chung Hyoung Doo Shin2 12 Jin Woo Song13 Dong Soon Kim13 Choon-Sik Park 1 and Young-Soo Shim6 14 Abstract Background Interleukin-8 IL-8 is a potent chemo-attractant cytokine responsible for neutrophil infiltration in lungs with idiopathic pulmonary fibrosis IPF . The IL-8 protein and mRNA expression are increased in the lung with IPF. We evaluated the effect of single nucleotide polymorphisms SNPs of the IL-8 gene on the risk of IPF. Methods One promoter rs4073T A and two intronic SNPs rs2227307T G and rs2227306C T of the IL-8 genes were genotyped in 237 subjects with IPF and 456 normal controls. Logistic regression analysis was applied to evaluate the association of these SNPs with IPF. IL-8 in BAL fluids was measured using a quantitative sandwich enzyme immunoassay and promoter activity was assessed using the luciferase reporter assay. Results The minor allele frequencies of rs4073T A and rs2227307T G were significantly lower in the 162 subjects with surgical biopsy-proven IPF and 75 subjects with clinical IPF compared with normal controls in the recessive model OR and p and respectively . The IL-8 protein concentration in BAL fluids significantly increased in 24 subjects with IPF compared with 14 controls p . Nine IPF subjects homozygous for the rs4073 T A common allele exhibited higher levels of the IL-8 protein compared with six .