tailieunhanh - Báo cáo y học: "Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" "Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. | Respiratory Research BioMed Central Open Access Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways Changsuk Moon Jeong Ran Han Hyun-Jung Park Jong Sik Hah and Jihee Lee Kang Address Department of Physiology Division of Cell Biology Ewha Medical Research Center School of Medicine Ewha Womans University Seoul Seoul Korea Email Changsuk Moon - 94cmoon@ Jeong Ran Han - hanjrjr@ Hyun-Jung Park - starking002@ Jong Sik Hah - jshah@ Jihee Lee Kang - jihee@ Corresponding author Published 9 March 2009 Received 23 September 2008 Respiratory Research 2009 10 18 doi 1465-9921-10-18 Accepted 9 March 2009 This article is available from http content 10 1 18 2009 Moon et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Synthetic peptides containing the RGD sequence inhibit integrin-related functions in different cell systems. Here we investigated the effects of synthetic Arg-Gly-Asp-Ser RGDS peptide on key inflammatory responses to intratracheal . lipopolysaccharide LPS treatment and on the integrin signaled mitogen-activated protein MAP kinase pathway during the development of acute lung injury. Methods Saline or LPS mg kg was administered . with or without a single dose of RGDS I or 5 mg kg . anti-av or anti-P3 mAb 5 mg kg . . Mice were sacrificed 4 or 24 h post-LPS. Results A pretreatment with RGDS inhibited LPS-induced increases in neutrophil and macrophage numbers total protein levels and TNF-a and MIP-2 levels and matrix metalloproteinase-9 activity in bronchoalveolar lavage BAL fluid at 4 or 24 h post-LPS treatment. RGDS .