tailieunhanh - Báo cáo y học: "Hypoxia regulates human lung fibroblast proliferation via p53-dependent and -independent pathways"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" "Hypoxia regulates human lung fibroblast proliferation via p53-dependent and -independent pathways. | Respiratory Research BioMed Central Open Access Hypoxia regulates human lung fibroblast proliferation via p53-dependent and -independent pathways Shiro Mizuno 1 Herman J Bogaard2 Norbert F Voelkel2 Yukihiro Umeda1 Maiko Kadowaki1 Shingo Ameshima1 Isamu Miyamori1 and Takeshi Ishizaki3 Address Third Department of Internal Medicine University of Fukui 23-3 Eiheiji-cho Matsuoka Yoshida-gun Fukui Japan 2Pulmonary and Critical Care Medicine Division Virginia Commonwealth University 1101 E Marshall St Sanger Hall 7th Floor Room 7-020 Richmond Virginia USA and 3Department of Fundamental Nursing University of Fukui 23-3 Eiheiji-cho Matsuoka Yoshida-gun Fukui Japan Email Shiro Mizuno - shirotan@ Herman J Bogaard - hjbogaard@ Norbert F Voelkel - nvoelkel@ Yukihiro Umeda - umada@ Maiko Kadowaki - maik@ Shingo Ameshima - ame@ Isamu Miyamori - miyamori@ Takeshi Ishizaki - takeshi@ Corresponding author Published 6 March 2009 Received 21 August 2008 Respiratory Research 2009 10 17 doi l465-992l-l0-l7 Accepted 6 March 2009 This article is available from http content l0 l l7 2009 Mizuno et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hypoxia induces the proliferation of lung fibroblasts in vivo and in vitro. However the subcellular interactions between hypoxia and expression of tumor suppressor p53 and cyclin-dependent kinase inhibitors p2l and p27 remain unclear. Methods Normal human lung fibroblasts NHLF were cultured in a hypoxic chamber or exposed to desferroxamine DFX . DNA synthesis was measured using bromodeoxyuridine incorporation and expression of p53 p2l and p27 was measured