tailieunhanh - Báo cáo y học: "Distribution of airway narrowing responses across generations and at branching points, assessed in vitro by anatomical optical coherence tomography"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:. | Noble et al. Respiratory Research 2010 11 9 http content 11 1 9 RESPIRATORY RESEARCH RESEARCH Open Access Distribution of airway narrowing responses across generations and at branching points assessed in vitro by anatomical optical coherence tomography Peter B Noble 1 Robert A McLaughlin3 Adrian R West2 Sven Becker3 Julian J Armstrong3 Peter K McFawn2 Peter R Eastwood4 5 6 David R Hillman5 6 David D Sampson3 Howard W Mitchell2 Abstract Background Previous histological and imaging studies have shown the presence of variability in the degree of bronchoconstriction of airways sampled at different locations in the lung . heterogeneity . Heterogeneity can occur at different airway generations and at branching points in the bronchial tree. Whilst heterogeneity has been detected by previous experimental approaches its spatial relationship either within or between airways is unknown. Methods In this study distribution of airway narrowing responses across a portion of the porcine bronchial tree was determined in vitro. The portion comprised contiguous airways spanning bronchial generations 3-11 including the associated side branches. We used a recent optical imaging technique anatomical optical coherence tomography to image the bronchial tree in three dimensions. Bronchoconstriction was produced by carbachol administered to either the adventitial or luminal surface of the airway. Luminal cross sectional area was measured before and at different time points after constriction to carbachol and airway narrowing calculated from the percent decrease in luminal cross sectional area. Results When administered to the adventitial surface the degree of airway narrowing was progressively increased from proximal to distal generations r to P to . This serial heterogeneity was also apparent when carbachol was administered via the lumen though it was less pronounced. In contrast airway narrowing was not different at side branches and was .