tailieunhanh - Báo cáo y học: " Inhibiting toll-like receptor 4 signaling ameliorates pulmonary fibrosis during acute lung injury induced by lipopolysaccharide: an experimental study"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Inhibiting toll-like receptor 4 signaling ameliorates pulmonary fibrosis during acute lung injury induced by lipopolysaccharide: an experimental study. | Respiratory Research BioMed Central Research Inhibiting toll-like receptor 4 signaling ameliorates pulmonary fibrosis during acute lung injury induced by lipopolysaccharide an experimental study ZhengYu He YeSen Zhu and Hong Jiang Address Department of Anesthesiology Ninth People s Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 China Email ZhengYu He - hzyyy@ YeSen Zhu - zhuyesenjy@ Hong Jiang - jianghonjy@ Corresponding author Open Access Published 18 December 2009 Received 5 August 2009 Respiratory Research 2009 10 126 doi l465-992l-l0-l26 Accepted 18 December 2009 This article is available from http content l0 l 126 2009 He et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Toll-like receptor 4 TLR4 is essential in lipopolysaccharide LPS -induced fibroblast activation and collagen secretion in vitro. However its effects on the process of lung fibroblast activation and fibrosis initiation during LPS induced acute lung injury ALI remain unknown. The goal of the present study was to determine the effect of inhibiting TLR4 on LPS-induced ALI and fibrosis in vivo. Methods The ALI model was established by intraperitoneal injection of LPS in mice. TLR4-small hairpin RNA shRNA lentivirus was injected intravenously into the mice to inhibit TLR4 expression. mRNA and protein levels were detected by real-time PCR and Western-blot analysis respectively. The contents of the C-terminal propeptide of type I procollagen PICP in bronchoalveolar lavage fluid BALF were detected by ELISA and the degree of fibrosis was detected by van Gieson collagen staining the hydroxyproline assay and alpha smooth muscle actin a-SMA .

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