tailieunhanh - Báo cáo y học: " Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD. | Winkler et al. Respiratory Research 2011 12 29 http content 12 1 29 RESPIRATORY RESEARCH RESEARCH Open Access Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD A ỉ I 1 r r 1r2t E IS-. I A 4- - f p. IL . - r r- u- ws s 3 t I ĩ I_I f I 71 A A I S-. I E D vr 3 I Crrsnn l z z l 1 2 l I r r l ri Iz-i 1 Carla WinKler Elena N Atochina-vasserman Olar HOlz Michael F Deers ven J trpenoecK Norbert Krug Stefan RoepcKe4 Gereon Lauer4 Martin Elmlinger4 Jens M Hohlfeld1 2 Abstract Background Pulmonary surfactant protein D SP-D is considered as a candidate biomarKer for the functional integrity of the lung and for disease progression which can be detected in serum. The origin of SP-D in serum and how serum concentrations are related to pulmonary concentrations under inflammatory conditions is still unclear. Methods In a cross-sectional study comprising non-smoKers n 10 young - n 10 elderly smokers n 20 and smoKers with COPD n 20 we simultaneously analysed pulmonary and serum SP-D levels with regard to pulmonary function exercise repeatability and its quaternary structure by native gel electrophoresis. Statistical comparisons were conducted by ANOVA and post-hoc testing for multiple comparisons repeatability was assessed by Bland-Altman analysis. Results In COPD median IQR pulmonary SP-D levels were lower 129 68 ng ml compared to smoKers young 299 190 elderly 296 158 ng ml p and non-smoKers 967 708 ng ml p . The opposite was observed in serum with higher concentrations in COPD 140 89 ng ml as compared to non-smoKers 76 47 ng ml p . SP-D levels were reproducible and correlated with the degree of airway obstruction in all smoKers. In addition smoKing lead to disruption of the quaternary structure. Conclusions Pulmonary and serum SP-D levels are stable marKers influenced by smoKing and related to airflow obstruction and disease state. Smaller subunits of pulmonary SP-D and the rapid increase of serum SP-D levels