tailieunhanh - Báo cáo khoa học: A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A

Signal transducers and activators of transcription (STATs) regulate a vari-ety of cellular functions, including differentiation and proliferation. STAT3 and STAT5 are known to play important roles in brain processes, such as energy homeostasis and neuronal development. We isolated a novel splicing variant ofSTAT5Afrom a cDNA library of the mouse brainstem. | A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A Yoshihisa Watanabe1 Masaya Ikegawa2 Yoshihisa Naruse3 and Masaki Tanaka1 1 Department of CellBiology Research Institute for NeurologicalDiseases and Geriatrics Kyoto PrefecturalUniversity of Medicine Japan 2 Department of Genomic MedicalSciences Kyoto PrefecturalUniversity of Medicine Japan 3 Department of Anatomy MedicalEducation and Research Center Meiji University of Integrative Medicine Kyoto Japan Keywords brainstem coaggregation STAT5A splicing variant suppression of STAT5A activity Correspondence M. Tanaka Department of CellBiology Research Institute for NeurologicalDiseases and Geriatrics Kyoto PrefecturalUniversity of Medicine Kawaramachi-Hirokoji Kamikyo-ku Kyoto 602-0841 Japan Tel Fax 81 75 251 5797 E-mail mtanaka@ Database The nucleotide sequence for the mouse STAT5A_DE18 cDNA has been submitted to the GenBank database under the accession number EU249543 Received 4 July 2009 revised 24 August 2009 accepted 1 September 2009 Signal transducers and activators of transcription STATs regulate a variety of cellular functions including differentiation and proliferation. STAT3 and STAT5 are known to play important roles in brain processes such as energy homeostasis and neuronal development. We isolated a novel splicing variant of STAT5A from a cDNA library of the mouse brainstem. This variant STAT5A_DE18 lacked exon 18 and caused a frameshift in the C-terminus resulting in deletion of a tyrosine phosphorylation site and a transactivation domain. Although the frameshift region had no characteristic motifs it was highly serine threonine-rich and contained a short proline-rich sequence. Expression of STAT5A_DE18 was detected in the mouse brainstem lung and thymus but not in the mouse cerebrum or cerebellum. We developed a specific antibody against STAT5A_AE18 and investigated the intracellular localization of this variant. .