tailieunhanh - Báo cáo khoa học: Rice cytosine DNA methyltransferases – gene expression profiling during reproductive development and abiotic stress

DNA methylation affects important developmental processes in both plants and animals. The process of methylation of cytosines at C-5 is catalysed by DNA methyltransferases (MTases), which are highly conserved, both struc-turally and functionally, in eukaryotes. In this study, we identified and characterized cytosine DNA MTase genes that are activated with the onset of reproductive development in rice. | Rice cytosine DNA methyltransferases - gene expression profiling during reproductive development and abiotic stress Rita Sharma1 z R. K. Mohan Singh1 z Garima Malik2 Priyanka Deveshwar1 Akhilesh K. Tyagi1 Sanjay Kapoor1 and Meenu Kapoor2 1 Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology University of Delhi South Campus New Delhi India 2 University Schoolof Biotechnology Guru Gobind Singh Indraprastha University Kashmere Gate Delhi India Keywords DNA methyltransferase epigenetics methylation microarray rice Oryza sativa Correspondence S. Kapoor Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology University of Delhi South Campus Benito Juarez Road New Delhi 110021 India Fax 91 11 24115095 Tel 91 9811644436 E-mail kapoors@ M. Kapoor University School of Biotechnology Guru Gobind Singh Indraprastha University Kashmere Gate Delhi 110006 India Fax 91 11 23900111 Tel 91 11 23900237 E-mail kapoorsk@ These authors contributed equally to this work DNA methylation affects important developmental processes in both plants and animals. The process of methylation of cytosines at C-5 is catalysed by DNA methyltransferases MTases which are highly conserved both structurally and functionally in eukaryotes. In this study we identified and characterized cytosine DNA MTase genes that are activated with the onset of reproductive development in rice. The rice genome Oryza sativa L. subsp. japonica encodes a total of 10 genes that contain the highly conserved MTase catalytic domain. These genes have been categorized into subfamilies on the basis of phylogenetic relationships. A microarray-based gene expression profile of all 10 MTases during 22 stages tissues that included 14 stages of reproductive development and five vegetative tissues together with three stresses cold salt and dehydration stress revealed specific windows of MTase activity during panicle and seed development. The .

• Báo cáo khoa học
• scientific reports
• thesis report
• medical report
• DNA methylation affects important developmental processes in both plants and animals
• The process of methylation of cytosines at C 5 is catalysed by DNA methyltransferases (MTases)
• which are highly conserved
• both struc turally and functionally
• in eukaryotes
• In this study
• we identified and characterized cytosine DNA MTase genes that are activated with the onset of reproductive development in rice