tailieunhanh - Báo cáo y học: " Differences in allergen-induced T cell activation between allergic asthma and rhinitis: Role of CD28, ICOS and CTLA-4"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Differences in allergen-induced T cell activation between allergic asthma and rhinitis: Role of CD28, ICOS and CTLA-4. | Botturi et al. Respiratory Research 2011 12 25 http content 12 1 25 RESPIRATORY RESEARCH RESEARCH Open Access Differences in allergen-induced T cell activation between allergic asthma and rhinitis Role of CD28 ICOS and CTLA-4 4 Karine Botturi Yannick Lacoeuille Arnaud Cavaillès Daniel Vervloet Antoine Magnan Abstract Background Th2 cell activation and T regulatory cell Treg deficiency are key features of allergy. This applies for asthma and rhinitis. However with a same atopic background some patients will develop rhinitis and asthma whereas others will display rhinitis only. Co-receptors are pivotal in determining the type of T cell activation but their role in allergic asthma and rhinitis has not been explored. Our objective was to assess whether allergen-induced T cell activation differs from allergic rhinitis to allergic rhinitis with asthma and explore the role of ICOS CD28 and CTLA-4. Methods T cell co-receptor and cytokine expressions were assessed by flow cytometry in PBMC from 18 house dust mite HDM allergic rhinitics R 18 HDM allergic rhinitics and asthmatics AR 13 non allergic asthmatics A and 20 controls with or without anti-co-receptors antibodies. Results In asthmatics A AR a constitutive decrease of CTLA-4 and of CD4 CD25 Foxp3 cells was found with an increase of IFN-y cells. In allergic subjects R AR allergen stimulation induced CD28 together with IL-4 and IL-13 and decreased the proportion of CTLA-4 IL-10 and CD4 CD25 Foxp3 cells. Anti-ICOS and anti-CD28 antibodies blocked allergen-induced IL-4 and IL-13. IL-13 production also involved CTLA-4. Conclusions T cell activation differs between allergic rhinitis and asthma. In asthma a constitutive co-receptor independent Th1 activation and Treg deficiency is found. In allergic rhinitis an allergen-induced Treg cell deficiency is seen as well as an ICOS- CD28- and CTLA-4-dependent Th2 activation. Allergic asthmatics display both characteristics. Background .