tailieunhanh - Báo cáo y học: " Placenta growth factor and vascular endothelial growth factor B expression in the hypoxic lung"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Placenta growth factor and vascular endothelial growth factor B expression in the hypoxic lung. | Sands et al. Respiratory Research 2011 12 17 http content 12 1 17 RESPIRATORY RESEARCH RESEARCH Open Access Placenta growth factor and vascular endothelial growth factor B expression in the hypoxic lung Michelle Sands Katherine Howell Christine M Costello Paul McLoughlin Abstract Background Chronic alveolar hypoxia due to residence at high altitude or chronic obstructive lung diseases leads to pulmonary hypertension which may be further complicated by right heart failure increasing morbidity and mortality. In the non-diseased lung angiogenesis occurs in chronic hypoxia and may act in a protective adaptive manner. To date little is known about the behaviour of individual vascular endothelial growth factor VEGF family ligands in hypoxia-induced pulmonary angiogenesis. The aim of this study was to examine the expression of placenta growth factor PlGF and VEGFB during the development of hypoxic pulmonary angiogenesis and their functional effects on the pulmonary endothelium. Methods Male Sprague Dawley rats were exposed to conditions of normoxia 21 O2 or hypoxia 10 O2 for 1-21 days. Stereological analysis of vascular structure real-time PCR analysis of vascular endothelial growth factor A VEGFA VEGFB placenta growth factor PlGF VEGF receptor 1 VEGFR1 and VEGFR2 immunohistochemistry and western blots were completed. The effects of VEGF ligands on human pulmonary microvascular endothelial cells were determined using a wound-healing assay. Results Typical vascular remodelling and angiogenesis were observed in the hypoxic lung. PlGF and VEGFB mRNA expression were significantly increased in the hypoxic lung. Immunohistochemical analysis showed reduced expression of VEGFB protein in hypoxia although PlGF protein was unchanged. The expression of VEGFA mRNA and protein was unchanged. In vitro PlGF at high concentration mimicked the wound-healing actions of VEGFA on pulmonary microvascular endothelial monolayers. Low concentrations of PlGF potentiated .