tailieunhanh - Báo cáo y học: " Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report. | Okubo et al. Journal of Medical Case Reports 2010 4 265 http content 4 1 265 jAl JOURNALOF medical ÌỤr case REPORTS CASE REPORT Open Access Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffin-embedded tissue for polymerase chain reaction a case report 11 2 1 11 Yoichiro Okubo Minoru Shinozaki Sadako Yoshizawa Haruo Nakayama Megumi Wakayama Tsutomu Hatori 1 1 11 1 Aki Mituda Takayuki Hirano Kayoko Shimodaira Zhi Yuzhu Kazutoshi Shibuya Abstract Introduction Toxoplasmosis can be a life-threatening disease when it occurs in patients with HIV infection. In particular meningioencephalitis has been regarded as the most common toxoplasmic complication in such patients. However toxoplasmic meningitis in a patient with HIV infection is extremely rare and purulent or tuberculous meningitis should be considered initially as a disease for differential diagnosis in Japan. Case presentation Toxoplasmic meningitis in a patient with HIV infection is reported. A 36-year-old Japanese man presented with fever pulsating headache lumbago nausea and vomiting. No examinations suggested toxoplasmosis including cerebrospinal fluid examinations images and serological tests. The result of a polymerase chain reaction assay using paraffin-embedded section was regarded as the conclusive evidence for the diagnosis. Conclusions We wish to emphasize the usefulness of polymerase chain reaction assays with nucleic acid extracted from paraffin-embedded tissue sections processed for routine histopathological examination if the section shows the infectious agents or findings suggesting some infectious diseases. Introduction Toxoplasma gondii is known as one of the most common infectious protozoan parasites that has a worldwide distribution 1-3 . Cats are recognized as the only definitive hosts of T. gondii but humans can be infected by the ingestion of oocysts or tissue cysts 4 . T. gondii infection is generally asymptomatic or associated