tailieunhanh - Báo cáo y học: "An ovine tracheal explant culture model for allergic airway inflammation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An ovine tracheal explant culture model for allergic airway inflammation. | Abeynaike et al. Journal of Inflammation 2010 7 46 http content 7 1 46 JOURNAL OF INFLAMMATION RESEARCH Open Access An ovine tracheal explant culture model for allergic airway inflammation Latasha Abeynaike Els NT Meeusen Robert J Bischof Abstract Background The airway epithelium is thought to play an important role in the pathogenesis of asthmatic disease. However much of our understanding of airway epithelial cell function in asthma has been derived from in vitro studies that may not accurately reflect the interactive cellular and molecular pathways active between different tissue constituents in vivo. Methods Using a sheep model of allergic asthma tracheal explants from normal sheep and allergic sheep exposed to house dust mite HDM allergen were established to investigate airway mucosal responses ex vivo. Explants were cultured for up to 48 h and tissues were stained to identify apoptotic cells goblet cells mast cells and eosinophils. The release of cytokines IL-1a IL-6 and TNF-a by cultured tracheal explants was assessed by ELISA. Results The general morphology and epithelial structure of the tracheal explants was well maintained in culture although evidence of advanced apoptosis within the mucosal layer was noted after culture for 48 h. The number of alcian blue PAS positive mucus-secreting cells within the epithelial layer was reduced in all cultured explants compared with pre-cultured 0 h explants but the loss of staining was most evident in allergic tissues. Mast cell and eosinophil numbers were elevated in the allergic tracheal tissues compared to naive controls and in the allergic tissues there was a significant decline in mast cells after 24 h culture in the presence or absence of HDM allergen. IL-6 was released by allergic tracheal explants in culture but was undetected in cultured control explants. Conclusions Sheep tracheal explants maintain characteristics of the airway mucosa that may not be replicated when studying .