tailieunhanh - Báo cáo y học: "Differential patterns of histone acetylation in inflammatory bowel diseases"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential patterns of histone acetylation in inflammatory bowel diseases. | Tsaprouni et al. Journal of Inflammation 2011 8 1 http content 8 1 1 JOURNAL OF INFLAMMATION RESEARCH Open Access Differential patterns of histone acetylation in inflammatory bowel diseases 11 3 1 2 Loukia G Tsaprouni Kazuhiro Ito Jonathan J Powell Ian M Adcock Neville Punchard Abstract Post-translational modifications of histones particularly acetylation are associated with the regulation of inflammatory gene expression. We used two animal models of inflammation of the bowel and biopsy samples from patients with Crohn s disease CD to study the expression of acetylated histones H 3 and 4 in inflamed mucosa. Acetylation of histone H4 was significantly elevated in the inflamed mucosa in the trinitrobenzene sulfonic acid model of colitis particularly on lysine residues K 8 and 12 in contrast to non-inflamed tissue. In addition acetylated H4 was localised to inflamed tissue and to Peyer s patches PP in dextran sulfate sodium DSS -treated rat models. Within the PP H3 acetylation was detected in the mantle zone whereas H4 acetylation was seen in both the periphery and the germinal centre. Finally acetylation of H4 was significantly upregulated in inflamed biopsies and PP from patients with CD. Enhanced acetylation of H4K5 and K16 was seen in the PP. These results demonstrate that histone acetylation is associated with inflammation and may provide a novel therapeutic target for mucosal inflammation. Introduction The cause of inflammatory bowel disease IBD remains unknown. The main forms of IBD are Crohn s disease and Ulcerative colitis. The main difference between Crohn s disease and UC is the location and nature of the inflammatory changes. Crohn s can affect any part of the gastrointestinal tract from mouth to anus skip lesions although a majority of the cases start in the terminal ileum. Ulcerative colitis in contrast is restricted to the colon and the rectum 1 . It has been proposed that epithelial abnormalities are the central defect and