tailieunhanh - Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children. | Casimir et al. Journal of Inflammation 2010 7 28 http content 7 1 28 JOURNAL OF INFLAMMATION RESEARCH Open Access Gender differences and inflammation an in vitro model of blood cells stimulation in prepubescent children Georges JA Casimir 1 Fabienne Heldenbergh1 Laurence Hanssens1 Sandra Mulier1 Claudine Heinrichs1 Nicolas Lefevre1 Julie Désir1 Francis Corazza2 and Jean Duchateau2 Abstract Background Gender influences clinical presentations and markers in inflammatory diseases. In many chronic conditions frequency of complications is greater in females suggesting that continuous inflammatory reaction may induce greater damage in targeted organs and functions. Methods To investigate gender dimorphism at a cellular level we evaluated the production of cytokines implicated in inflammatory processes IL -1 IL- 6 PGE-2 and TNF alpha in healthy prepubescent children of both sex and Turner s syndrome TS patients genotype XO . We used stimulation by LPS and 1 ng ml and Pokeweed Mitogen PWM on overnight cultures from whole blood samples collected in 57 subjects 22 girls 26 boys 5-96 months and 9 TS patients 6-15 years . The primary outcome was to evaluate if gender influences the production of cytokines with potential relation to X chromosome monosomy. Secondary endpoints were to relate different cytokines level productions and conditions. Results We confirm the male over female increased cytokine productions already observed in adults. This is contrasting with numerous observations obtained in vivo about increased production of inflammatory markers in females CRP ESR and neutrophil counts as we recently reported in children. Relative variations of the dimorphism according to stimulus its concentration and cytokine type are discussed presenting IL6 with a modulating function that could be more potent in males. TS subjects follow mostly the male pattern of reactivity sustaining the role of some gene expression differing with X chromosome .