tailieunhanh - Báo cáo y học: "Treatment with apolipoprotein A-1 mimetic peptide reduces lupus-like manifestations in a murine lupus model of accelerated atherosclerosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Treatment with apolipoprotein A-1 mimetic peptide reduces lupus-like manifestations in a murine lupus model of accelerated atherosclerosis. | Woo et al. Arthritis Research Therapy 2010 12 R93 http content 12 3 R93 RESEARCH ARTICLE Open Access Treatment with apolipoprotein A-1 mimetic peptide reduces lupus-like manifestations in a murine lupus model of accelerated atherosclerosis Jennifer MP Woo 1 Zhuofeng Lin1 Mohamad Navab2 Casey Van Dyck1 Yvette Trejo-Lopez1 Krystal MT Woo1 Hongyun Li1 Lawrence W Castellani3 Xuping Wang3 Noriko Iikuni1 Ornella J Rullo4 Hui Wu1 Antonio La Cava1 Alan M Fogelman5 Aldons J Lusis2 and Betty P Tsao 1 Abstract Introduction The purpose of this study was to evaluate the effects of L-4F an apolipoprotein A-1 mimetic peptide alone or with pravastatin in apoE- -Fas- -C57BL 6 mice that spontaneously develop immunoglobulin G IgG autoantibodies glomerulonephritis osteopenia and atherosclerotic lesions on a normal chow diet. Methods Female mice starting at eight to nine weeks of age were treated for 27 weeks with 1 pravastatin 2 L-4F 3 L-4F plus pravastatin or 4 vehicle control followed by disease phenotype assessment. Results In preliminary studies dysfunctional proinflammatory high-density lipoproteins piHDL were decreased six hours after a single L-4F but not scrambled L-4F injection in eight- to nine-week old mice. After 35 weeks L-4F-treated mice in the absence presence of pravastatin had significantly smaller lymph nodes and glomerular tufts PL LP lower serum levels of IgG antibodies to double stranded DNA dsDNA PL and oxidized phospholipids oxPLs PL LP and elevated total and vertebral bone mineral density PL LP compared to vehicle controls. Although all treatment groups presented larger aortic root lesions compared to vehicle controls enlarged atheromas in combination treatment mice had significantly less infiltrated CD68 macrophages PLP significantly increased mean a-actin stained area PLP and significantly lower levels of circulating markers for atherosclerosis progression CCL19 PL LP and VCAM-1 PL . .