tailieunhanh - Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus. | Nie et al. Arthritis Research Therapy 2010 12 R91 http content 12 3 R91 RESEARCH ARTICLE Open Access Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus Ying J Nie 1 Mo Y Mok1 Godfrey CF Chan2 Albert W Chan1 Ou Jin1 Sushma Kavikondala1 Albert KW Lie1 and ChakSLau 1 Abstract Introduction Systemic lupus erythematosus SLE is an autoimmune disease characterized by autoreactive T and B cells which are believed to be secondary to deficient dendritic cells DCs . However whether DC abnormalities occur during their development in the bone marrow BM or in the periphery is not known. Methods Thirteen patients with SLE and 16 normal controls were recruited. We studied the morphology phenotype and functional abilities of bone marrow-derived dendritic cells BMDCs generated by using two culture methods FMS-like tyrosine kinase 3 Flt3 -ligand FL and granulocyte-macrophage colony-stimulating factor GM-CSF plus interleukin-4 IL-4 respectively. Results BMDCs induced by FL exhibited both myeloid mDC and plasmacytoid DC pDC features whereas GM-CSF IL-4 induced mDC generation. Substantial phenotypic and functional defects of BMDCs were found from patients with SLE at different stages of cell maturation. When compared with healthy controls SLE immature BM FLDCs expressed higher levels of CCR7. Both immature and mature SLE BM FLDCs expressed higher levels of CD40 and CD86 and induced stronger T-cell proliferation. SLE BM mDCs expressed higher levels of CD40 and CD86 but lower levels of HLA-DR and a lower ability to stimulate T-cell proliferation when compared with control BM mDCs. Conclusions Our data are in accordance with previous reports that suggest that DCs have a potential pathogenic role in SLE. Defects of these cells are evident during their development in BM. BM mDCs are deficient whereas BM pDCs which are part of BM FLDCs are the likely culprit in inducing autoimmunity in SLE. Introduction Systemic