tailieunhanh - Báo cáo y học: "Regulation of IFN response gene activity during infliximab treatment in rheumatoid arthritis is associated with clinical response to treatment"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Regulation of IFN response gene activity during infliximab treatment in rheumatoid arthritis is associated with clinical response to treatment. | van Baarsen et al. Arthritis Research Therapy 2010 12 R11 http content 12 1 R11 RESEARCH ARTICLE Open Access Regulation of IFN response gene activity during infliximab treatment in rheumatoid arthritis is associated with clinical response to treatment Lisa GM van Baarsen 1 2 Carla A Wijbrandts3 Francois Rustenburg1 Tineke Cantaert3 Tineke CTM van der Pouw Kraan4 Dominique L Baeten3 Ben AC Dijkmans5 Paul PTak3 and Cornelis L Verweij 1 5 Abstract Introduction Cross-regulation between TNF and type I IFN has been postulated to play an important role in autoimmune diseases. Therefore we determined the effect of TNF blockade in rheumatoid arthritis RA on the type I IFN response gene activity in relation to clinical response. Methods Peripheral blood from 33 RA patients was collected in PAXgene tubes before and after the start of infliximab treatment. In a first group of 15 patients the baseline expression of type I IFN-regulated genes was determined using cDNA microarrays and compared to levels one month after treatment. The remaining 18 patients were studied as an independent group for validation using quantitative polymerase chain reaction qPCR . Results Gene expression analysis revealed that anti-TNF antibody treatment induced a significant increase in type I IFN response gene activity in a subset of RA patients whereas expression levels remained similar or were slightly decreased in others. The findings appear clinically relevant since patients with an increased IFN response gene activity after anti-TNF therapy had a poor clinical outcome. This association was confirmed and extended for an IFN response gene set consisting of OAS1 LGALS3BP Mx2 OAS2 and SERPING1 in five EULAR good and five EULAR poor responders by qPCR. Conclusions Regulation of IFN response gene activity upon TNF blockade in RA is not as consistent as previously described but varies between patients. The differential changes in IFN response gene activity appear relevant to the .