tailieunhanh - Báo cáo y học: " Cytotoxic T cells expressing the co-stimulatory receptor NKG2 D are increased in cigarette smoking and COPD"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Cytotoxic T cells expressing the co-stimulatory receptor NKG2 D are increased in cigarette smoking and COPD. | Roos-Engstrand et al. Respiratory Research 2010 11 128 http content 11 1 128 RESPIRATORY RESEARCH RESEARCH Open Access Cytotoxic T cells expressing the co-stimulatory receptor NKG2 D are increased in cigarette smoking and COPD 1 1 13 Ester Roos-Engstrand Jamshid Pourazar Annelie F Behndig Anders Blomberg Anders Bucht Abstract Background A suggested role for T cells in COPD pathogenesis is based on associations between increased lung cytotoxic T lymphocyte CD8 numbers and airflow limitation. CD69 is an early T cell activation marker. Natural Killer cell group 2 D NKG2D receptors are co-stimulatory molecules induced on CD8 T cells upon activation. The activating function of NKG2 D is triggered by binding to MHC class 1 chain-related MIC molecules A and B expressed on surface of stressed epithelial cells. The aim of this study was to evaluate the expression of MIC A and B in the bronchial epithelium and NKG2 D and CD69 on BAL lymphocytes in subjects with COPD compared to smokers with normal lung function and healthy never-smokers. Methods Bronchoscopy with airway lavages and endobronchial mucosal biopsy sampling was performed in 35 patients with COPD 21 healthy never-smokers and 16 smokers with normal lung function. Biopsies were immunohistochemically stained and BAL lymphocyte subsets were determined using flow cytometry. Results Epithelial CD3 lymphocytes in bronchial biopsies were increased in both smokers with normal lung function and in COPD patients compared to never-smokers. Epithelial CD8 lymphocyte numbers were higher in the COPD group compared to never-smoking controls. Among gated CD3 cells in BAL the percentage of CD8 NKG2D cells was enhanced in patients with COPD and smokers with normal lung function compared to never-smokers. The percentage of CD8 CD69 cells and cell surface expression of CD69 were enhanced in patients with COPD and smokers with normal lung function compared to never-smokers. No changes in the expression