tailieunhanh - Báo cáo y học: "Influence of cytokine inhibitors on concentration and activity of MMP-1 and MMP-3 in disc herniation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Influence of cytokine inhibitors on concentration and activity of MMP-1 and MMP-3 in disc herniation. | Available online http content 11 6 R169 Open Access Research article Influence of cytokine inhibitors on concentration and activity of MMP-1 and MMP-3 in disc herniation Stéphane Genevay1 Axel Finckh1 Franpoise Mezin1 Enrico Tessitore2 and Pierre-André Guerne1 Division of Rheumatology University Hospitals of Geneva Avenue Beau-Séjour 26 1211 Geneva 14 Switzerland 2Division of Neurosurgery University Hospitals of Geneva rue Gabrielle-Perret-Gentil 4 1211 Geneva 14 Switzerland Corresponding author Stéphane Genevay Received 27 Mar 2009 Revisions requested 13 May 2009 Revisions received 18 Sep 2009 Accepted 11 Nov 2009 Published 11 Nov 2009 Arthritis Research Therapy 2009 11 R169 doi 86 ar2858 This article is online at http content 11 6 R169 2009 Genevay et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Spontaneous resorption of disc herniation DH after sciatica is well documented. The matrix metalloproteinases MMP -1 and MMP-3 are enzymes potentially involved in this process. Glucocorticoid injections are commonly used for treatment and other anti-inflammatory molecules like tumor necrosis factor TNF inhibitors are under clinical investigation. However little is known about the effect of these molecules on DH resorption. Methods DH tissue was harvested from patients undergoing surgery for sciatica. Samples were thoroughly washed. Diced explants were cultured ex-vivo in 1 ml Dulbecco s modified Eagle s medium DMEM 10 fetal calf serum FCS controls 2 recombinant interleukin 1 receptor antagonist IL-1Ra 100 ng ml 3 dexamethasone 10E-5 M or 4 TNF inhibitor monoclonal antibody 10 pg ml . Supernatants were harvested at 48 .

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