tailieunhanh - Báo cáo y học: " Respiratory Syncytial Virus (RSV) RNA loads in peripheral blood correlates with disease severity in mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Respiratory Syncytial Virus (RSV) RNA loads in peripheral blood correlates with disease severity in mice. | Torres et al. Respiratory Research 2010 11 125 http content 11 1 125 RESPIRATORY RESEARCH RESEARCH Open Access Respiratory Syncytial Virus RSV RNA loads in peripheral blood correlates with disease severity in mice 12 13 1 1 Juan Pablo Torres Ana M Gomez Shama Khokhar Vijay G Bhoj Claudia Tagliabue Michael L Chang Dofor A IZionor4 I IH Ạ Do oll2 D milrn5 Aci mrinn Í ÌOÍÌ2C1 5 Peter A Kienei Paula A Revell Octavio Ramilo Asuncion Mejias Abstract Background Respiratory Syncytial Virus RSV infection is usually restricted to the respiratory epithelium. Few studies have documented the presence of RSV in the systemic circulation however there is no consistent information whether virus detection in the blood correlates with disease severity. Methods Balb c mice were inoculated with live RSV heat-inactivated RSV or medium. A subset of RSV-infected mice was treated with anti-RSV antibody 72 h post-inoculation. RSV RNA loads were measured by PCR in peripheral blood from day 1-21 post-inoculation and were correlated with upper and lower respiratory tract viral loads the systemic cytokine response lung inflammation and pulmonary function. Immunohistochemical staining was used to define the localization of RSV antigens in the respiratory tract and peripheral blood. Results RSV RNA loads were detected in peripheral blood from day 1 to 14 post-inoculation peaked on day 5 and significantly correlated with nasal and lung RSV loads airway obstruction and blood CCL2 and CXCL1 expression. Treatment with anti-RSV antibody reduced blood RSV RNA loads and improved airway obstruction. Immunostaining identified RSV antigens in alveolar macrophages and peripheral blood monocytes. Conclusions RSV RNA was detected in peripheral blood upon infection with live RSV followed a time-course parallel to viral loads assessed in the respiratory tract and was significantly correlated with RSV-induced airway disease. Background RSV is the most common cause of acute lower .