tailieunhanh - Báo cáo y học: "Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells. | Ghosh et al. Arthritis Research Therapy 2010 12 R28 http content 12 1 R28 RESEARCH ARTICLE Open Access Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells Peter Ghosh1 2 Jiehua Wu 1 Susan Shimmon1 Andrew CW Zannettino3 Stan Gronthos4 Silviu Itescu2 Abstract Introduction This study was undertaken to determine whether the anti-osteoarthritis drug pentosan polysulfate PPS influenced mesenchymal precursor cell MPC proliferation and differentiation. Methods Human MPCs were maintained in monolayer pellet or micromass cultures MMC for up to 10 days with PPS at concentrations of 0 to 20 pg ml. MPC viability and proliferation was assessed using the WST-1 assay and 3H-thymidine incorporation into DNA while apoptosis was monitored by flow cytometry. Proteoglycan PG biosynthesis was determined by 35SO42- incorporation and staining with Alcian blue. Proteoglycan and collagen type I and collagen type II deposition in pellet cultures was also examined by Toluidine blue and immunohistochemical staining respectively. The production of hyaluronan HA by MPCs in MMC was assessed by ELISA. The relative outcome of PPS HA heparin or dextran sulfate DS on PG synthesis was compared in 5-day MMC. Gene expression of MPCs in 7-day and 10-day MMC was examined using real-time PCR. MPC differentiation was investigated by co-culturing with PPS in osteogenic or adipogenic inductive culture media for 28 days. Results Significant MPC proliferation was evident by day 3 at PPS concentrations of 1 to 5 pg ml P . In the presence of 1 to 10 pg ml PPS a 38 reduction in IL-4 IFNy-induced MPC apoptosis was observed. In 5-day MMC 130 stimulation of PG synthesis occurred at pg ml PPS P while pg ml PPS achieved maximal stimulation in the 7-day and 10-day cultures P . HA and DS at 5 pg ml inhibited PG synthesis P in 5-day MMC. Collagen type II deposition by MMC was .