tailieunhanh - Báo cáo y học: " Hemoglobin a and b are ubiquitous in the human lung, decline in idiopathic pulmonary fibrosis but not in COPD"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Hemoglobin a and b are ubiquitous in the human lung, decline in idiopathic pulmonary fibrosis but not in COPD. | Ishikawa et al. Respiratory Research 2010 11 123 http content 11 1 123 RESPIRATORY RESEARCH RESEARCH Open Access Hemoglobin a and b are ubiquitous in the human lung decline in idiopathic pulmonary fibrosis but not in COPD 3 4 151 Nobuhisa Ishikawa 1 Steffen Ohlmeier Kaisa Salmenkivi Marjukka Myllarniemi Irfan Rahman Witold Mazur Vuokko L Kinnula1 Abstract Background Idiopathic pulmonary fibrosis IPF and chronic obstructive pulmonary disease COPD are disorders of the lung parenchyma. They share the common denominators of a progressive nature and poor prognosis. The goal was to use non-biased proteomics to discover new markers for these diseases. Methods Proteomics of fibrotic vs. control lung tissue suggested decreased levels of several spots in the lung specimens of IPF patients which were identified as Hemoglobin Hb a and b monomers and Hba complexes. The Hba and b monomers and complexes were investigated in more detail in normal lung and lung specimens of patients with IPF and COPD by immunohistochemistry morphometry and mass spectrometry MS . Results Both Hb monomers in normal lung were expressed especially in the alveolar epithelium. Levels of Hba and b monomers and complexes were reduced lost in IPF but not in the COPD lungs when compared to control lung. MS-analyses revealed Hba modification at cysteine105 Cysa105 preventing formation of the Hba complexes in the IPF lungs. Hba and Hbb were expressed as complexes and monomers in the lung tissues but were secreted into the bronchoalveolar lavage fluid and or induced sputum supernatants as complexes corresponding to the molecular weight of the Hb tetramer. Conclusions The abundant expression of the oxygen carrier molecule Hb in the normal lung epithelium and its decline in IPF lung are new findings. The loss of Hb complex formation in IPF warrants further studies and may be considered as a disease-specific modification. Background Idiopathic pulmonary fibrosis IPF histopathology of .