tailieunhanh - Báo cáo y học: " Allergic inflammation does not impact chemical-induced carcinogenesis in the lungs of mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Allergic inflammation does not impact chemical-induced carcinogenesis in the lungs of mice. | Doris et al. Respiratory Research 2010 11 118 http content 11 1 118 RESPIRATORY RESEARCH RESEARCH Open Access Allergic inflammation does not impact chemical-induced carcinogenesis in the lungs of mice 1 11 11 Konstantinos Doris Sophia P Karabela Chrysoula A Kairi Davina CM Simoes Charis Roussos 1 12 3 134 Spyros G Zakynthinos Ioannis Kalomenidis Timothy S Blackwell Georgios T Stathopoulos Abstract Background Although the relationship between allergic inflammation and lung carcinogenesis is not clearly defined several reports suggest an increased incidence of lung cancer in patients with asthma. We aimed at determining the functional impact of allergic inflammation on chemical carcinogenesis in the lungs of mice. Methods Balb c mice received single-dose urethane 1 g kg at day 0 and two-stage ovalbumin during tumor initiation sensitization days -14 and 0 challenge daily at days 6-12 tumor progression sensitization days 70 and 84 challenge daily at days 90-96 or chronically sensitization days -14 and 0 challenge daily at days 6-12 and thrice weekly thereafter . In addition interleukin IL -5 deficient and wild-type C57BL 6 mice received ten weekly urethane injections. All mice were sacrificed after four months. Primary end-points were number size and histology of lung tumors. Secondary end-points were inflammatory cells and mediators in the airspace compartment. Results Ovalbumin provoked acute allergic inflammation and chronic remodeling of murine airways evident by airspace eosinophilia IL-5 up-regulation and airspace enlargement. Urethane resulted in formation of atypical alveolar hyperplasias adenomas and adenocarcinomas in mouse lungs. Ovalbumin-induced allergic inflammation during tumor initiation progression or continuously did not impact the number size or histologic distribution of urethane-induced pulmonary neoplastic lesions. In addition genetic deficiency in IL-5 had no effect on urethane-induced lung tumorigenesis. Conclusions .