tailieunhanh - Báo cáo y học: "Differential expression level of cytokeratin 8 in cells of the bovine nucleus pulposus complicates the search for specific intervertebral disc cell markers"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Differential expression level of cytokeratin 8 in cells of the bovine nucleus pulposus complicates the search for specific intervertebral disc cell markers. | Gilson et al. Arthritis Research Therapy 2010 12 R24 http content 12 1 R24 RESEARCH ARTICLE Open Access Differential expression level of cytokeratin 8 in cells of the bovine nucleus pulposus complicates the search for specific intervertebral disc cell markers Audrey Gilson 1 Mathias Dreger2 and Jill PG Urban 1 Abstract Introduction Development of cell therapies for repairing the intervertebral disc is limited by the lack of a source of healthy human disc cells. Stem cells particularly mesenchymal stem cells are seen as a potential source but differentiation strategies are limited by the lack of specific markers that can distinguish disc cells from articular chondrocytes. Methods We searched for markers using the differential in-gel electrophoresis proteomic technology to compare proteins of bovine nucleus pulposus cells phenotypically similar to mature human nucleus cells with those of bovine articular chondrocytes. In the cohort of the differentially expressed proteins identified by mass spectrometry cytokeratin 8 CK8 was further validated by immunostaining of freshly isolated cells and frozen tissue sections using monoclonal antibodies. Results We identified a set of 14 differentially expressed proteins. Immunohistochemistry showed that only a subset of cells approximately 10 was positive for one of these proteins CK8 an intermediate filament protein present in epithelial but not mesenchymal cells. In tissue sections CK8-positive cells were seen in all discs examined and appeared as small isolated clusters surrounded by gelatinous matrix. Notochordal nucleus pulposus cells from pig phenotypically similar to human infant nucleus pulposus cells were all CK8-positive. The mesenchymal intermediate filament protein vimentin was present in all bovine and porcine nucleus pulposus cells. Conclusions The notochordal cell population is reported to disappear from the nucleus pulposus of bovine discs before birth and from human discs in childhood. .

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