tailieunhanh - Báo cáo y học: " The early asthmatic response is associated with glycolysis, calcium binding and mitochondria activity as revealed by proteomic analysis in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:The early asthmatic response is associated with glycolysis, calcium binding and mitochondria activity as revealed by proteomic analysis in rats. | Xu et al. Respiratory Research 2010 11 107 http content 11 1 107 RESPIRATORY RESEARCH RESEARCH Open Access The early asthmatic response is associated with glycolysis calcium binding and mitochondria activity as revealed by proteomic analysis in rats X P s z 1 1 2 2 1 2 X X 1 Yu-Dong Xu Jian-Mei Cui Yu Wang Lei-Miao Yin Chang-Ke Gao Yan-Yan Liu Yong-Qing Yang Abstract Background The inhalation of allergens by allergic asthmatics results in the early asthmatic response EAR which is characterized by acute airway obstruction beginning within a few minutes. The EAR is the earliest indicator of the pathological progression of allergic asthma. Because the molecular mechanism underlying the EAR is not fully defined this study will contribute to a better understanding of asthma. Methods In order to gain insight into the molecular basis of the EAR we examined changes in protein expression patterns in the lung tissue of asthmatic rats during the EAR using 2-DE MS-based proteomic techniques. Bioinformatic analysis of the proteomic data was then performed using PPI Spider and KEGG Spider to investigate the underlying molecular mechanism. Results In total 44 differentially expressed protein spots were detected in the 2-DE gels. Of these 44 protein spots 42 corresponded to 36 unique proteins successfully identified using mass spectrometry. During subsequent bioinformatic analysis the gene ontology classification the protein-protein interaction networking and the biological pathway exploration demonstrated that the identified proteins were mainly involved in glycolysis calcium binding and mitochondrial activity. Using western blot and semi-quantitative RT-PCR we confirmed the changes in expression of five selected proteins which further supports our proteomic and bioinformatic analyses. Conclusions Our results reveal that the allergen-induced EAR in asthmatic rats is associated with glycolysis calcium binding and mitochondrial activity which could .