tailieunhanh - Báo cáo y học: "SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling. | Chu et al. Respiratory Research 2010 11 155 http content 11 1 155 RESPIRATORY RESEARCH RESEARCH Open Access SPLUNC1 regulation in airway epithelial cells role of toll-like receptor 2 signaling 12 1 1 3 1 71 2 Hong Wei Chu Fabienne Gally Jyoti Thaikoottathil Yvonne M Janssen-Heininger Qun Wu Gongyi Zhang 2 1 111 1 1 Nichole Reisdorph Stephanie Case Maisha Minor Sean Smith Di Jiang Nicole Michels Glenn Simon Richard J Martin1 Abstract Background Respiratory infections including Mycoplasma pneumoniae Mp contribute to various chronic lung diseases. We have shown that mouse short palate lung and nasal epithelium clone 1 SPLUNC1 protein was able to inhibit Mp growth. Further airway epithelial cells increased SPLUNC1 expression upon Mp infection. However the mechanisms underlying SPLUNC1 regulation remain unknown. In the current study we investigated if SPLUNC1 production following Mp infection is regulated through Toll-like receptor 2 TLR2 signaling. Methods Airway epithelial cell cultures were utilized to reveal the contribution of TLR2 signaling including NF-kB to SPLUNC1 production upon bacterial infection and TLR2 agonist stimulation. Results Mp and TLR2 agonist Pam3CSK4 increased SPLUNC1 expression in tracheal epithelial cells from wild type but not TLR2- - BALB c mice. RNA interference short-hairpin RNA of TLR2 in normal human bronchial epithelial cells under air-liquid interface cultures significantly reduced SPLUNC1 levels in Mp-infected or Pam3CSK4-treated cells. Inhibition and activation of NF-kB pathway decreased and increased SPLUNC1 production in airway epithelial cells respectively. Conclusions Our data for the first time suggest that airway epithelial TLR2 signaling is pivotal in mycoplasma-induced SPLUNC1 production thus improving our understanding of the aberrant SPLUNC1 expression in airways of patients suffering from chronic lung diseases with bacterial infections. Background Palate lung and nasal epithelium clone PLUNC are a .