tailieunhanh - Báo cáo y học: "NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigen­specific CD4+ T cell polarization"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigen­specific CD4+ T cell polarization. | Hodgkins et al. Respiratory Research 2010 11 102 http content 11 1 102 RESPIRATORY RESEARCH RESEARCH Open Access NO2 inhalation induces maturation of pulmonary CD11c cells that promote antigen-specific CD4 T cell polarization 1111 1 Samantha R Hodgkins Jennifer L Ather Sara A Paveglio Jenna L Allard Laurie A Whittaker LeClair Benjamin T Suratt1 Jonathan E Boyson2 Matthew E Poynter1 Abstract Background Nitrogen dioxide NO2 is an air pollutant associated with poor respiratory health asthma exacerbation and an increased likelihood of inhalational allergies. NO2 is also produced endogenously in the lung during acute inflammatory responses. NO2 can function as an adjuvant allowing for allergic sensitization to an innocuous inhaled antigen and the generation of an antigen-specific Th2 immune response manifesting in an allergic asthma phenotype. As CD11c antigen presenting cells are considered critical for naive T cell activation we investigated the role of CD11c cells in NO2-promoted allergic sensitization. Methods We systemically depleted CD11c cells from transgenic mice expressing a simian diphtheria toxin DT receptor under of control of the CD11c promoter by administration of DT. Mice were then exposed to 15 ppm NO2 followed by aerosolized ovalbumin to promote allergic sensitization to ovalbumin and were studied after subsequent inhaled ovalbumin challenges for manifestation of allergic airway disease. In addition pulmonary CD11c cells from wildtype mice were studied after exposure to NO2 and ovalbumin for cellular phenotype by flow cytometry and in vitro cytokine production. Results Transient depletion of CD11c cells during sensitization attenuated airway eosinophilia during allergen challenge and reduced Th2 and Th17 cytokine production. Lung CD11c cells from wildtype mice exhibited a significant increase in MHCII CD40 and OX40L expression 2 hours following NO2 exposure. By 48 hours CD11c MHCII DCs within the mediastinal lymph node MLN .