tailieunhanh - Báo cáo y học: "Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients. | Urbanowicz et al. Respiratory Research 2010 11 76 http content 11 1 76 RESPIRATORY RESEARCH RESEARCH Open Access Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients Richard A Urbanowicz1 Jonathan R Lamb2 Ian Todd1 Jonathan M Corne3 Lucy C Fairclough1 Abstract Background We have previously shown that NK CD56 CD3- and NKT-like CD56 CD3 cells are reduced in both numbers and cytotoxicity in peripheral blood. The aim of the present study was to investigate their numbers and function within induced sputum. Methods Induced sputum cell numbers and intracellular granzyme B and perforin were analysed by flow cytometry. Immunomagnetically selected CD56 cells NK and NKT-like cells were used in an LDH release assay to determine cytotoxicity. Results The proportion of NK cells and NKT-like cells in smokers with COPD COPD subjects was significantly higher and 3 respectively than in healthy smokers smokers p 1 p and nonsmoking healthy subjects HNS p p . The proportions of NK cells and NKT-like cells expressing both perforin and granzyme B were also significantly higher in COPD subjects compared to smokers and HNS. CD56 cells from COPD subjects were significantly more cytotoxic 1414 biological lytic activity than those from smokers p and HNS p and were inversely correlated to FEV1. r p . Conclusion We have shown an increased proportion of NK and NKT-like cells in the induced sputum of COPD subjects and have demonstrated that these cells are significantly more cytotoxic in COPD subjects than smokers and HNS. Background Chronic obstructive pulmonary disease COPD is a complex condition consisting of emphysema respiratory bronchiolitis and chronic bronchitis 1 . It is projected to be the fifth commonest cause of morbidity and the third leading cause of death worldwide by 2030 2 . Tobacco smoking is established as the main aetiological factor for COPD and it