tailieunhanh - Báo cáo y học: " Inhaled salmeterol and/or fluticasone alters structure/function in a murine model of allergic airways disease"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Inhaled salmeterol and/or fluticasone alters structure/function in a murine model of allergic airways disease. | Riesenfeld et al. Respiratory Research 2010 11 22 http content 11 1 22 RESPIRATORY RESEARCH RESEARCH Open Access Inhaled salmeterol and or fluticasone alters structure function in a murine model of allergic airways disease Erik P Riesenfeld Michael J Sullivan John A Thompson-Figueroa Hans C Haverkamp Lennart K Lundblad Jason HT Bates Charles G Irvin Abstract Background The relationship between airway structural changes remodeling and airways hyperresponsiveness AHR is unclear. Asthma guidelines suggest treating persistent asthma with inhaled corticosteroids and long acting p-agonists LABA . We examined the link between physiological function and structural changes following treatment fluticasone and salmeterol separately or in combination in a mouse model of allergic asthma. Methods BALB c mice were sensitized to intraperitoneal ovalbumin OVA followed by six daily inhalation exposures. Treatments included 9 daily nebulized administrations of fluticasone alone 6 mg ml salmeterol 3 mg ml or the combination fluticasone and salmeterol. Lung impedance was measured following methacholine inhalation challenge. Airway inflammation epithelial injury mucus containing cells and collagen content were assessed 48 hours after OVA challenge. Lungs were imaged using micro-CT. Results and Discussion Treatment of allergic airways disease with fluticasone alone or in combination with salmeterol reduced AHR to approximately nauve levels while salmeterol alone increased elastance by 39 compared to control. Fluticasone alone and fluticasone in combination with salmeterol both reduced inflammation to near naive levels. Mucin containing cells were also reduced with fluticasone and fluticasone in combination with salmeterol. Conclusions Fluticasone alone and in combination with salmeterol reduces airway inflammation and remodeling but salmeterol alone worsens AHR and these functional changes are consistent with the concomitant changes in mucus metaplasia. .