tailieunhanh - Báo cáo y học: "Modulation of cytokine and nitric oxide by mesenchymal stem cell transfer in lung injury/fibrosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Modulation of cytokine and nitric oxide by mesenchymal stem cell transfer in lung injury/fibrosis. | Lee et al. Respiratory Research 2010 11 16 http content 11 1 16 RESPIRATORY RESEARCH RESEARCH Open Access Modulation of cytokine and nitric oxide by mesenchymal stem cell transfer in lung injury fibrosis Shin-Hwa Leet1 An-Soo Jangt1 Young-Eun Kim 1 Ji-Yeon Cha1 Tae-Hoon Kim1 Seok Jung1 Seong-Kyu Park2 You-Kyoung Lee3 Jong-Ho Won4 Yong-Hoon Kim 5 and Choon-Sik Park 1 Abstract Background No effective treatment for acute lung injury and fibrosis currently exists. Aim of this study was to investigate the time-dependent effect of bone marrow-derived mesenchymal stem cells BMDMSCs on bleomycin BLM -induced acute lung injury and fibrosis and nitric oxide metabolites and inflammatory cytokine production. Methods BMDMSCs were transferred 4 days after BLM inhalation. Wet dry ratio bronchoalveolar lavage cell profiles histologic changes and deposition of collagen were analyzed. Results Nitrite nitrate and cytokines were measured weekly through day 28. At day 7 the wet dry ratio neutrophilic inflammation and amount of collagen were elevated in BLM-treated rats compared to sham rats p . Levels nitrite nitrate IL-1P IL-6 TNF-a TGF-P and VEGF were also higher at day 7 p . Degree of lymphocyte and macrophage infiltration increased steadily over time. BMDMSC transfer significantly reduced the BLM-induced increase in wet dry ratio degree of neutrophilic infiltration collagen deposition and levels of the cytokines nitrite and nitrate to those in sham-treated rats p . Fluorescence in situ hybridization localized the engrafted cells to areas of lung injury. Conclusion Systemic transfer of BMDMSCs effectively reduced the BLM-induced lung injury and fibrosis through the down-regulation of nitric oxide metabolites and proinflammatory and angiogenic cytokines. Background Acute lung injury ALI is characterized by diffuse alveolar injury profound inflammation increased vascular permeability and alveolar flooding which together may result in acute