tailieunhanh - Báo cáo y học: "Cigarette smoke promotes dendritic cell accumulation in COPD; a Lung Tissue Research Consortium study"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Cigarette smoke promotes dendritic cell accumulation in COPD; a Lung Tissue Research Consortium study. | Vassallo et al. Respiratory Research 2010 11 45 http content 11 1 45 RESPIRATORY RESEARCH RESEARCH Open Access Cigarette smoke promotes dendritic cell accumulation in COPD a Lung Tissue Research Consortium study Robert Vassallo 1 Paula R Walters1 Jeffrey Lamont1 Theodore J Kottom1 Eunhee S Yi1 2 and Andrew H Limper 1 3 Abstract Background Abnormal immune responses are believed to be highly relevant in the pathogenesis of chronic obstructive pulmonary disease COPD . Dendritic cells provide a critical checkpoint for immunity by their capacity to both induce and suppress immunity. Although evident that cigarette smoke the primary cause of COPD significantly influences dendritic cell functions little is known about the roles of dendritic cells in the pathogenesis of COPD. Methods The extent of dendritic cell infiltration in COPD tissue specimens was determined using immunohistochemical localization of CD83 cells marker of matured myeloid dendritic cells and CD1a cells Langerhans cells . The extent of tissue infiltration with Langerhans cells was also determined by the relative expression of the CD207 gene in COPD versus control tissues. To determine mechanisms by which dendritic cells accumulate in COPD complimentary studies were conducted using monocyte-derived human dendritic cells exposed to cigarette smoke extract CSE and dendritic cells extracted from mice chronically exposed to cigarette smoke. Results In human COPD lung tissue we detected a significant increase in the total number of CD83 cells and significantly higher amounts of CD207 mRNA when compared with control tissue. Human monocyte-derived dendritic cells exposed to CSE exhibited enhanced survival in vitro when compared with control dendritic cells. Murine dendritic cells extracted from mice exposed to cigarette smoke for 4 weeks also demonstrated enhanced survival compared to dendritic cells extracted from control mice. Acute exposure of human dendritic cells to CSE .

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