tailieunhanh - Cytokines Genes Polymorphisms in Iranian Patients with Pulmonary Tuberculosis

Mycobacteria are endowed with mechanisms through which they can evade the onslaught ofhost defense response. These mechanisms are discussed including diminishing the ability ofantigen presenting cells to present antigens to CD4+ T cells; production of suppressive cytokines;escape from fused phagosomes and inducing T cell review brings out the complexity of the host-pathogen interaction and underlines theimportance of identifying the mechanisms involved in protection, in order to design vaccinestrategies and find out surrogate markers to be measured as in vitro correlate of protectiveimmunity | Cytokines Genes Polymorphisms in Iranian Patients with Pulmonary Tuberculosis Ali Akbar Amirzargar Abdol Ali Danesh Farideh Khosravi Mohammad Hossein Niknam Behrouz Nikbin Immunogenetic Laboratory Department of Immunology School of Medicine Tehran University of Medical Sciences Tehran Iran. ABSTRACT Background Pulmonary tuberculosis PTB has recently become a major problem in developed countries especially in immune compromised HIV infected individuals. Cytokines their genes and receptors have been implicated in the protective immunity I pathophysiology and development of tuberculosis. Material Methods In the present study the genotype frequencies of a number of polymorphic genes coding for cytokines or for cytokine receptors have been investigated in a case control study including a group of 40 Iranian PTB patients and 40 healthy individuals. The allelic polymorphism I of cytokines SNPs were analyzed according to the protocols of the cytokine component designed for the 13th IHW by the Heidelberg University group. Using PCR-SSP method the following cytokine genes have been determined IL-1a T C -889 IL-1P C T 3962 IL-1R C T pstI 1970 IL-1RA T C mspaI 1100 IL-4RA G A 19o2 IL-12 C A -1188 TGF-p C T codon 10 G C codon 25 TNF-a G A -308 G A -238 IL-2 T G -330 G T 166 IL-4 T G -1098 T C -590 T C -33 IL-6 G C -174 G A nt 560 IL-10 G A -1082 C T -819 C A -592 . Results From IL-1R cluster pro- inflammatory cytokines a positive significant association was found at position pstI 1970 C T polymorphism where the C allele was over presented in the PTB patients 60 vs. P . A significant negative association at codon 10 TGF-P C T polymorphism has also been shown in our patients where the T allele was not detected in the patients but 10 of the control subjects expressed this allele Fisher exact test I P . At this codon allele T Leucine substitution is associated with high TGF-P production. For TNFa an insignificant tendency was found at position -308 A G polymorphism .