tailieunhanh - SPECIFICITY OF SPUTUM SMEAR COMPARED TO CULTURE IN DIAGNOSIS OF PULMONARY TUBERCULOSIS

The role of macrophage apoptosis in containing the growth ofthe bacilli is also discussed. The role of other components of innate immune response such asnatural resistance associated macrophage protein (Nramp), neutrophils, and natural killer cellshas been discussed. The specific acquired immune response through CD4 T cells, mainlyresponsible for protective Th1 cytokines and through CD8 cells bringing about cytotoxicity,also has been described. The role of CD-1 restricted CD8+ T cells and non-MHC restricted γγγγγ/δδδδδT cells has been described although it is incompletely understood at the present time. Humoralimmune response is seen though not implicated in protection. The value of cytokine therapyhas. | World Journal of Medical Sciences 6 3 121-125 2011 ISSN 1817-3055 IDO SI Publications 2011 Specificity of Sputum Smear Compared to Culture in Diagnosis of Pulmonary tuberculosis . Nour . Saeed 2Ahmed . Zaki and3ElNageeb s. Saeed 1 Ministry of Animal Resources and Fisheries Sudan zFaculty of Veterinary Medicine University of Khartoum Sudan 3Faculty of Medicine University of Khartoum Sudan Abstract The specificity of sputum smear in comparison with culture in diagnosis of pulmonary tuberculosis was determined. Three hundred and twenty nine smear-positive sputum samples were collected from 3 tuberculosis clinics in Khartoum State Sudan decontaminated and cultured onto Loowenste in-Jensen medium. Growth was monitored daily during the first week for the presence of rapidly growing mycobacteria nocardiae or other acid-fast organisms and then weekly for up to 8 weeks for the presence of Mycobacterium tuberculosis complex MTC . The isolates with microscopic and cultural properties consistent with MTC were confirmed by biochemical testing and IS6110-PCR. The percentage of agreement between the sputum smear and culture was . The rest of the samples revealed either Nocardia species rapidly growing mycobacteria found to be contaminated or showed no growth. This study indicated that acid-fast bacilli such as Nocardia spp. and rapidly growing mycobacteria should be considered when smear method is used to diagnose pulmonary tuberculosis and a confirmatoiy diagnostic method such as sputum culture or PCR is necessary to avoid the implications of mistreatment. Key words specificity sputum smear Mycobacterium tuberculosis Rapidly growing mycobacteria Nocardia INTRODUCTION Diagnosis of tuberculosis in the developing countries where over 90 of TB cases occur 1 is based primarily on symptoms microscopic examination of smears for acid-fast bacilli AFB and occasionally on chest x-ray 2 Slide microscopy is a convenient rapid and economical test used for .

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