tailieunhanh - báo cáo khoa hoc : EphrinA5 suppresses colon cancer development by negatively regulating epidermal growth factor receptor stability

Colon cancer is one of the most common human cancers worldwide. Owing to its aggressiveness and lethality, it is necessary to determine the mecha-nisms regulating the carcinogenesis of colon cancer. EphrinA5 has been reported to act as a putative tumor suppressor in glioma; however, little is known concerning the role of this protein in the context of colon cancer. | IFEBS Journal EphrinA5 suppresses colon cancer development by negatively regulating epidermal growth factor receptor stability Tong-Hong Wang1 Junn-Liang Chang2 3 Jar-Yi Ho4 Hsiao-Chun Wu1 and Tse-Ching Chen1 5 1 Tissue Bank Chang Gung Memorial Hospital Tao-Yuan Taiwan 2 Department of Pathology Laboratory Medicine Taoyuan Armed Forces General Hospital Tao-Yuan Taiwan 3 BiomedicalEngineering Department Ming Chuang University Tao-Yuan Taiwan 4 Graduate Institute of Life Sciences NationalDefense MedicalCenter Taipei Taiwan 5 Department of Anatomic Pathology Chang Gung Memorial Hospital Chang Gung University Schoolof Medicine Tao-Yuan Taiwan Keywords carcinogenesis colon cancer ephrinA5 epidermal growth factor receptor EGFR tumor suppressor Correspondence . Chen Department of Anatomic Pathology Chang Gung Memorial Hospital 5 Fu-Shin St Kwei-Shan Tao-Yuan 333 Taiwan Fax 886 3 3280147 Tel 886 3 3281200 ext. 2730 E-mail ctc323@ These authors contributed equally to this work Received 6 July 2011 revised 4 October 2011 accepted 26 October 2011 doi Colon cancer is one of the most common human cancers worldwide. Owing to its aggressiveness and lethality it is necessary to determine the mechanisms regulating the carcinogenesis of colon cancer. EphrinA5 has been reported to act as a putative tumor suppressor in glioma however little is known concerning the role of this protein in the context of colon cancer. To elucidate the biological significance of ephrinA5 in colon cancer we examined ephrinA5 and epidermal growth factor receptor EGFR expression profiles in both colon cancer and normal tissues using immunohistochemistry on a 96-spot tissue microarray. Gain-of-function and loss-of-function experiments were performed on the human colon cancer cell lines SW480 and WiDr to determine the biological effects of ephrinA5 in relation to cell proliferation survival and migration. It was found that ephrinA5 mRNA and protein levels .