tailieunhanh - Mass Spectrometry 2

If you read March’s paper on ion traps: – What is resonant excitation? Summarize how resonant excitation is used in typical ion trap MS experiments. If you read the Russell and Edmondson paper on MALDITOF and accurate mass: – Summarize the advantages and disadvantages of MALDI-TOF (with DE and reflection) versus FTICR (including ESI-FTICR), especially in biochemical applications. If you read the Aeberold and Goodlett proteomics paper: – Why is MS used so heavily in the study of post-translational modifications? Briefly describe an application to phosphopeptide sequence determinations | VILLANOVA UNIVERSITY Mass Spectrometry and Related Techniques 2 Lecture Date February 27th 2008 Ion and Particle Spectrometry 2 - Outline Atomic and Molecular Mass Spectrometry - Skoog et al. Ch 11 and 20. Please read this additional reference - R. Aebersold and D. R. Goodlett Mass Spectrometry in Proteomics Chem. Rev. 2001 101 269-295. Ion Mobility Spectrometry - If interested see G. W. Eiceman Critical Rev. Anal. Chem. 1991 22 471-489. D. C. Collins and M. L. Lee Developments in ion mobility spectrometry -mass spectrometry Anal. Bioanal. Chem. 2002 372 66-73. 1 Homework Problems If you read March s paper on ion traps - What is resonant excitation Summarize how resonant excitation is used in typical ion trap MS experiments. If you read the Russell and Edmondson paper on MALDI-TOF and accurate mass - Summarize the advantages and disadvantages of MALDI-TOF with DE and reflection versus FTICR including ESI-FTICR especially in biochemical applications. If you read the Aeberold and Goodlett proteomics paper - Why is MS used so heavily in the study of post-translational modifications Briefly describe an application to phosphopeptide sequence determinations. If you read the Sleno and Volmer ion activation methods paper - Pick any two of the ion activation processes described in the paper . in Table 1 describe how it works and the approximate energies involved and list one advantage Applications of Mass Spectrometry Interpretation of mass spectra is the key to most applications of the technique Information contained in a mass spectrum - Molecular weight via exact or mono-isotopic mass . Usually obtained though a suitably accurate measurement of M - the molecular ion an odd-electron species M H and M-H the protonated de-protonated molecule an even-electron species In some techniques can be confirmed by M Na M K M NH4 dimers trimers and other adducts etc. - Molecular formula - Ionization energies - Isotopic incorporation ex. 13C 14C 2H 3H. - Fragmentation and ion .

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