tailieunhanh - báo cáo khoa học: "mRNA analysis of single living cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: mRNA analysis of single living cells | Journal of Nanobiotechnology BioMed Central Research mRNA analysis of single living cells Toshiya Osada Hironori Uehara Hyonchol Kim and Atsushi Ikai Open Access Address Department of Life Science Graduate School of Bioscience and Biotechnology Tokyo Institute of Technology Nagatsuta Midori-ku Yokohama 226-8501 Japan Email Toshiya Osada - tosada@ Hironori Uehara - huehara@ Hyonchol Kim - hkim@ Atsushi Ikai - aikai@ Corresponding author Published 14 February 2003 Received 3 December 2002 Journal of Nanobiotechnology 2003 1 2 Accepted 14 February 2003 This article is available from http content 1 1 2 2003 Osada et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Analysis of specific gene expression in single living cells may become an important technique for cell biology. So far no method has been available to detect mRNA in living cells without killing or destroying them. We have developed here a novel method to examine gene expression of living cells using an atomic force microscope AFM . AFM tip was inserted into living cells to extract mRNAs. The obtained mRNAs were analyzed with RT-PCR nested PCR and quantitative PCR. This method enabled us to examine time-dependent gene expression of single living cells without serious damage to the cells. Background The properties of individual cells depend on molecules that constitute them. The different combinations of protein expression result in structural and functional changes of individual cells. Although most of these events depend on differences in gene expression no method is available to examine time dependent gene expression of individual living cells. There are other methods to analyze mRNA from single cells. For example the cellular .