tailieunhanh - báo cáo khoa học: "Self-assembly of proteins and their nucleic acids"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Self-assembly of proteins and their nucleic acids | Journal of Nanobiotechnology BioMed Central Research Self-assembly of proteins and their nucleic acids Graham Fletcher Sean Mason Jon Terrett and Mikhail Soloviev Open Access Address Oxford GlycoSciences UK Ltd Abingdon Oxon OX14 3YS United Kingdom Email Graham Fletcher - Sean Mason - Jon Terrett - Mikhail Soloviev - Corresponding author Published 28 January 2003 Received 25 November 2002 Accepted 28 January 2003 Journal of Nanobiotechnology 2003 1 1 This article is available from http content 1 1 1 2003 Fletcher et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract We have developed an artificial protein scaffold herewith called a protein vector which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical linkage between a functional protein and its genetic code. The principle is demonstrated using a streptavidin-based protein vector SAPV as both a nucleic acid binding pocket and a protein display system. We have shown that functional proteins or protein domains can be produced in vitro and physically linked to their DNA in a single enzymatic reaction. Such self-assembled protein-DNA complexes can be used for protein cloning the cloning of protein affinity reagents or for the production of proteins which selfassemble on a variety of solid supports. Self-assembly can be utilised for making libraries of protein-DNA complexes or for labelling the protein part of such a complex to a high specific activity by labelling the nucleic acid associated with the protein. In summary self-assembly offers an opportunity to quickly generate cheap protein .