tailieunhanh - Báo cáo khoa học: Nigrocin-2 peptides from Chinese Odorrana frogs – integration of UPLC/MS/MS with molecular cloning in amphibian skin peptidome analysis

Peptidomics is a powerful set of tools for the identification, structural eluci-dation and discovery of novel regulatory peptides and for monitoring the degradation pathways of structurally and catalytically important proteins. Amphibian skin secretions, arising from specialized granular glands, often contain complex peptidomes containing many components of entirely novel structure and unique site-substituted analogues of known peptide families. | ỊFEBS Journal Nigrocin-2 peptides from Chinese Odorrana frogs -integration of UPLC MS MS with molecular cloning in amphibian skin peptidome analysis Lei Wang1 Geisa Evaristo2 Mei Zhou1 Martijn Pinkse2 4 Min Wang1 Ying Xu1 Xiaofeng Jiang1 Tianbao Chen1 Pingfan Rao3 Peter Verhaert2 4 5 and Chris Shaw1 1 Molecular Therapeutics Research Schoolof Pharmacy Queen s University Belfast Northern Ireland 2 Department of Biotechnology Kluyver Laboratory Delft University of Technology Delft The Netherlands 3 Institute of Biotechnology Fuzhou University Fujian Province China 4 Netherlands Proteomics Centre Delft The Netherlands 5 Flemish Institute of Biotechnology Laboratory for Molecular CellBiology Leuven Belgium Keywords amphibian antimicrobial mass spectrometry molecular cloning peptide Correspondence C. Shaw Schoolof Pharmacy Queen s University 97 Lisburn Road Belfast BT9 7BL Northern Ireland UK Fax 44 2890 247794 Tel 44 2890 972129 E-mail Database The nucleotide sequences of seven nigrocins named nigrocin-2N nigrocin-2HJ nigrocin-2VB nigrocin-2LVa and b and nigrocin-2SCa-c from the skin of Chinese frogs Pelophylax nigromaculatus Odorrana hejiangensis Odorrana versabilis Odorrana livida and Odorrana schmackeri repesctively have submitted to the EMBL nucleotide sequence database under accession numbers AM494062 AM493894 FM160679 AM295098 AM295099 AM494476 FM160677 and FM60678 respectively Received 12 November 2009 revised 5 January 2010 accepted 12 January 2010 doi Peptidomics is a powerful set of tools for the identification structural elucidation and discovery of novel regulatory peptides and for monitoring the degradation pathways of structurally and catalytically important proteins. Amphibian skin secretions arising from specialized granular glands often contain complex peptidomes containing many components of entirely novel structure and unique site-substituted analogues of known peptide families. Following the .