tailieunhanh - Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo

Lipocalin-type prostaglandin (PG) D synthase is expressed in adipose tissues and involved in the regulation of glucose tolerance and atherosclero-sis in type 2 diabetes. However, the physiological roles of PGD2 in adipo-genesisin vivo are not clear, as lipocalin-type prostaglandin D synthase can also act as a transporter for lipophilic molecules, such as retinoids. | Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo Yasushi Fujitani1 Kosuke Aritake1 Yoshihide Kanaoka1 2 Tsuyoshi Goto3 Nobuyuki Takahashi3 Ko Fujimori1 4 and Teruo Kawada3 1 Department of Molecular BehavioralBiology Osaka Bioscience Institute Japan 2 Department of Medicine Harvard Medical School Division of Rheumatology Immunology and Allergy Brigham and Women s Hospital Boston MA USA 3 Laboratory of Molecular Function of Food Division of Food Science and Biotechnology Graduate Schoolof Agriculture Kyoto University Japan 4 Laboratory of Biodefense and Regulation Osaka University of PharmaceuticalSciences Japan Keywords adipocytes H-PGDS obesity PGD2 transgenic mouse Correspondence K. Fujimori Laboratory of Biodefense and Regulation Osaka University of PharmaceuticalSciences 4-20-1 Nasahara Takatsuki Osaka 569-1094 Japan Fax 81 726 690 1055 Tel 81 726 690 1055 E-mail fujimori@ Present address PharmaceuticalResearch Division Takeda Pharmaceutical Co. Ltd. Osaka Japan Received 28 October 2009 revised 22 December 2009 accepted 4 January 2010 doi Lipocalin-type prostaglandin PG D synthase is expressed in adipose tissues and involved in the regulation of glucose tolerance and atherosclerosis in type 2 diabetes. However the physiological roles of PGD2 in adipogenesis in vivo are not clear as lipocalin-type prostaglandin D synthase can also act as a transporter for lipophilic molecules such as retinoids. We generated transgenic TG mice overexpressing human hematopoietic PGDS H-PGDS and investigated the in vivo functions of PGD2 in adipogenesis. PGD2 production in white adipose tissue of H-PGDS TG mice was increased approximately seven-fold as compared with that in wild-type WT mice. With a high-fat diet H-PGDS TG mice gained more body weight than WT mice. Serum leptin and insulin levels were increased in H-PGDS TG mice and the triglyceride level was decreased by .