tailieunhanh - báo cáo khoa học: "Nanoscaled carborane ruthenium(II)-arene complex inducing lung cancer cells apoptosis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Nanoscaled carborane ruthenium(II)-arene complex inducing lung cancer cells apoptosis | Zhang et al. Journal of Nanobiotechnology 2011 9 6 http content 9 1 6 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Nanoscaled carborane ruthenium II -arene complex inducing lung cancer cells apoptosis Gen Zhang1 Chunhui Wu1 Hongde Ye2 Hong Yan2 Xuemei Wang1 Abstract Background The new ruthenium II -arene complex which bearing a carborane unit ruthenium and ferrocenyl functional groups has a novel versatile synthetic chemistry and unique properties of the respective material at the nanoscale level. The ruthenium II -arene complex shows significant cytotoxicity to cancer cells and tumor-inhibiting properties. However ruthenium II -arene complex of mechanism of anticancer activity are scarcely explored. Therefore it is necessary to explore ruthenium II -arene complex mechanism of anticancer activity for application in this area. Results In this study the ruthenium II -arene complex could significantly induce apoptosis in human lung cancer HCC827 cell line. At the concentration range of 5 gM-100 gM ruthenium II -arene complex had obvious cell cytotoxicity effect on HCC827 cells with IC50 values ranging gM. Additionally our observations demonstrate that the ruthenium II -arene complex can readily induce apoptosis in HCC827 cells as evidenced by Annexin-V-FITC nuclear fragmentation as well as DNA fragmentation. Treatment of HCC827 cells with the ruthenium II -arene complex resulted in dose-dependent cell apoptosis as indicated by high cleaved Caspase-8 9 ratio. Besides ruthenium II -arene complex caused a rapid induction of cleaved Caspase-3 activity and stimulated proteolytic cleavage of poly- ADP-ribose polymerase PARP in vitro and in vivo. Conclusion In this study the ruthenium II -arene complex could significantly induce apoptosis in human lung cancer HCC827 cell line. Treatment of HCC827 cells with the ruthenium II -arene complex resulted in dosedependent cell apoptosis as indicated by high cleaved Caspase-8 9 ratio. Besides